I, Proximity ligation assay of GBM0913 shPTGFRN or shGFP cells; negative control contained no primary antibody; positive control comprised two different antibodies targeting PTGFRN; * p<0.05 by student’s t test (above). For example, KIF11 and KIF15 play roles in generating and maintaining a bipolar spindle, CENPE in chromosome congression, kinesin-4 and kinesin-8 motors in microtubule regulation, and kinesin-6 motors in the central spindle and cytokinesis (Rath and Kozielski, 2012). GeneRIFs: Gene References Into Functions. The relatively small number of cadherin superfamily proteins encoded in the C. elegans genome, combined with its well-known transparency, simplicity of organization, and robust forward and reverse genetics makes C. elegans an appealing model organism for understanding how cadherins and their binding partners function in defined cellular contexts. 0000002632 00000 n Purification of His6-tagged zinc-finger protein YY1 by Ni- and Zn-IMAC. Different kinesins in this large superfamily are responsible for anterograde transport of specific cargo types within axons.  |  The 7/8S globulins, also termed vicilins, are somewhat simpler, comprising three subunits of ~40–80 kDa, but typically about 50 kDa. ARM repeat superfamily protein; FUNCTIONS IN: ubiquitin-protein ligase activity, binding, zinc ion binding; INVOLVED IN: protein ubiquitination; LOCATED IN: ubiquitin ligase complex; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Zinc … At3g22142. GST-pi (GSTP1) has been shown to be resistant to cisplatin, BCNU, cyclophosphamide, busulfan, and melphalan in cancer cell lines [104,105].

A, B, C, Western blot evaluating basal P-AKT…, PTGFRN promotes DNA damage sensing. N. Hirokawa, R. Takemura, in Encyclopedia of Biological Chemistry (Second Edition), 2013. Well-characterized BAR domain proteins and their functions.

2015 Aug 15;580:64-74. doi: 10.1016/j.abb.2015.07.001. Many BAR domain superfamily proteins contain additional domains that regulate their intracellular functions. Since that time, the sequences in the PIR-International Protein Sequence Database have been classified into protein superfamilies. Using this basic structural motif, a diverse range of biological functions have been derived, including sporulation proteins in fungi, sucrose-binding activities and enzymatic activities found in germins, where manganese is bound in the center of the barrel. trailer << /Size 73 /Info 30 0 R /Root 46 0 R /Prev 50020 /ID[<1c9d46f653c8f08e1932e5d994109475><1c9d46f653c8f08e1932e5d994109475>] >> startxref 0 %%EOF 46 0 obj << /Type /Catalog /Pages 29 0 R /Names 47 0 R /StructTreeRoot 53 0 R /SpiderInfo 36 0 R /Outlines 49 0 R /PageMode /UseOutlines >> endobj 47 0 obj << /IDS 34 0 R /URLS 35 0 R >> endobj 71 0 obj << /S 113 /O 176 /Filter /FlateDecode /Length 72 0 R >> stream KHC stalk regions contain the dimerization domains, while the C-terminal tails interact with ‘light chains’ (kinesin light chain, KLC) that confer cargo binding (Marx et al., 2006). A protein family is a group of proteins that share a common evolutionary origin, reflected by their related functions and similarities in sequence or structure. RING/U-box superfamily protein. Harpaz Y, Chothia C. Many of the immunoglobulin superfamily domains in cell adhesion molecules and surface receptors belong to a new structural set which is close to that containing variable domains[J]. Nucl. Dialyzed proteins and fresh dialysis buffer were analyzed for nickel and zinc content by ICPMS at Wessling Laboratories (Bochum, Germany) and the values determined for the buffer subtracted from the protein analyses. 0000001389 00000 n J. Mol. Conti S, Vexler A, Edry-Botzer L, Kalich-Philosoph L, Corn BW, Shtraus N, Meir Y, Hagoel L, Shtabsky A, Marmor S, Earon G, Lev-Ari S. PLoS One. Jeff Hardin, ... Jonathan Pettitt, in Progress in Molecular Biology and Translational Science, 2013. A, Western blot of GBM0913 shRNA cells that received either no treatment (NT) or radiation (10 Gy) and collected after 30 minutes. In flowering plants the cupin barrel has been duplicated to give the bi-cupins, which include the 7S and 11S seed storage globulins.

The terms superfamily (describing a large group of distantly related proteins) and subfamily (describing a small group of closely related proteins) are sometimes used in this context. These mitotic kinesins (kinesin spindle proteins; KSPs; Figure 5) have been considered as major targets for anticancer drugs because cancer cells possess infinite proliferative capacity. Keywords: ARM repeat superfamily protein NP_176225.1. We discuss it and other cadherins with known functions in the following sections. Katja Faelber, ... Oliver Daumke, in Progress in Molecular Biology and Translational Science, 2013. We discuss it and other cadherins with known functions in the following sections. Recently, overexpression of MGST2 and treatment of busulfan have been shown to increase cell survival in the fibroblast cell line. 0000006407 00000 n Table 1. Arabidopsis thaliana (Mouse-ear cress) Status. Protein concentration of elution fractions were determined by the Bradford method to 0.3 mg/ml (6.7 μM, Ni-NTA) and 0.25 mg/ml (5.6 μM, Zn-NTA). NTA resin was treated with ZnCl2 as described below to generate Zn-charged NTA (Zn-NTA). The CDPK-SnRK superfamily consists of seven types of serine-threonine protein kinases: calcium-dependent protein kinase (CDPKs), CDPK-related kinases (CRKs), phosphoenolpyruvate carboxylase kinases (PPCKs), PEP carboxylase kinase-related kinases (PEPRKs), calmodulin-dependent protein kinases (CaMKs), calcium and calmodulin-dependent protein kinases (CCaMKs), and SnRKs. KIF1Bα transports mitochondria, while mitochondria have another redundant motor KIF5. 0000006429 00000 n D, E, Western blot assessing p110β protein levels in GBM0821 and GBM0913 neurosphere cell lines. first published online November 16, 2018. https://doi.org/10.1093/nar/gky1130. Immunoblot of cells used for the PLA assay (below). Divergence, under a given selection pressure to retain a function, can create clusters of sequences that possess the same, or very similar, function (functional families). E. N. Clare Mills, ... Yuri Alexeev, in Food Allergy, 2012. Intuitively, sequences that form robust clusters in an SSN may therefore share the same or similar function(s).

Each subunit is synthesized in the seed as a single chain of ~60 kDa, which is post-translationally processed to give rise to acidic (~40 kDa) and basic (~20 kDa) chains, linked by a single disulfide bond, and are rarely, if ever, glycosylated. GST proteins exist throughout the cells, from cytosol to mitochondria to microsomes. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. URL: https://www.sciencedirect.com/science/article/pii/S0076687919300965, URL: https://www.sciencedirect.com/science/article/pii/B978012378630200431X, URL: https://www.sciencedirect.com/science/article/pii/S0076687909630275, URL: https://www.sciencedirect.com/science/article/pii/B9780123944474200503, URL: https://www.sciencedirect.com/science/article/pii/B9781437719925000028, URL: https://www.sciencedirect.com/science/article/pii/B9780123944474200606, URL: https://www.sciencedirect.com/science/article/pii/B9780123942951000263, URL: https://www.sciencedirect.com/science/article/pii/B9781416058953100099, URL: https://www.sciencedirect.com/science/article/pii/B978012394311800011X, URL: https://www.sciencedirect.com/science/article/pii/B9780123944474300335, Encyclopedia of Biological Chemistry (Second Edition), 2013, Janine N. Copp, ... Nobuhiko Tokuriki, in, Natarajan, Jendroszek, Kumar, et al., 2018, Encyclopedia of Biological Chemistry (Second Edition), Guide to Protein Purification, 2nd Edition, Lundmark and Carlsson (2004); Worby and Dixon (2002); Yarar, Beemon (1981); Greer (2002); Brooks-Wilson. C. elegans has 12 genes encoding 13 cadherins.1–3 C. elegans has representatives of most of the main cadherin families that are conserved throughout the metazoa, including members of the classical cadherin, Fat-like cadherin, Dachsous, Flamingo/CELSR, and Calsytenin families (Fig. © The SUPERFAMILY authors and Julian Gough 2001-2020. We use cookies to help provide and enhance our service and tailor content and ads.