We develop methods to label RNA in fixed and living cells using fluorescent probes and develop microscopy techniques and image analysis algorithms to visualize and quantify many mRNAs simultaneously. Dr. Robert H. Singer received his undergraduate degree in physical chemistry from Oberlin College and his Ph.D. in developmental biology from Brandeis University.

Dr. Singer seeks to follow the expression and movement of mRNA from transcription through degradation in live cells. He is Co-Director of the Gruss Lipper Biophotonics Center and a Senior Fellow of the Janelia Farm Research Campus of the HHMI. The research of Robert Singer, Ph.D., and colleagues has illuminated our understanding of gene activation, or transcription. Dr. Singer is professor and co-chair of anatomy & structural biology, the Harold and Muriel Block Chair in Anatomy & Structural Biology, and co-director of the Gruss-Lipper Biophotonics Center. His lab has shown that the dynamics of RNA transcription on a single gene can be interrogated by live cell imaging, as well as by multiplexed fluorescent probes. All rights reserved. He holds 12 patents on his work. He is a member of the National Academy of Sciences, American Society of Arts and Sciences and the Association for the Advancement of Science. An in situ hybridization technique his lab developed revealed that messenger RNA localizes in specific cellular compartments.

He is Co-Director of the Gruss Lipper Biophotonics Center and a Senior Fellow of the Janelia Farm Research Campus of the HHMI. 718.430.2000, Department of Anatomy & Structural Biology, Dominick P. Purpura Department of Neuroscience, A Novel Approach to Identifying Partners of RNA-binding Proteins, Random Gene Expression is Critical for Stem Cell Function, In Scientific First, Researchers Visualize Proteins Being Born, Master of Science in Clinical Research Methods (M.S. This work has given rise to the field of RNA transport and localization, enhanced by Dr. Singer’s and his colleagues’ development of imaging technology and RNA reporters.

). What Does it Look Like to 'Turn On' a Gene? Because these techniques yield quantitative fluorescence data, mathematical modeling can be applied to test mechanistic hypotheses about the kinetics of each of the events in an mRNA's life. He did postdoctoral work at MIT and at the Weizmann Institute. He received his undergraduate degree in physical chemistry from Oberlin College and his PhD in developmental biology from Brandeis University. Professor, Department of Anatomy & Structural Biology, Professor, Dominick P. Purpura Department of Neuroscience, Co-Chair Department of Anatomy & Structural Biology, Harold and Muriel Block Chair in Anatomy & Structural Biology, Co-Director, Gruss-Lipper Biophotonics Center, Albert Einstein College of MedicineJack and Pearl Resnick Campus1300 Morris Park AvenueGolding Building, Room 601Bronx, NY 10461, More Resources: Singer Laboratory Web PageASB Department Web Page, Is this your profile? This technology has implications for understanding of the role of RNA in disease processes such as cancer metastasis and mental retardation. His career has been focused on the cell biology of RNA: its isolation, detection, expression and translation. Because these techniques yield quantitative fluorescence data, we are able to apply mathematical modeling to test mechanistic hypotheses. Robert H. Singer is the Harold and Muriel Block Chair of Anatomy of Structural Biology, a Professor of Cell Biology and Professor of Neuroscience. A patented in situ hybridization technique his lab developed for detecting RNA in morphologically preserved cells revealed that messenger RNA can localize in specific cellular compartments. His career has been focused on the cell biology of RNA, its isolation, detection, expression and translation. This work has given rise to the field of RNA localization, enhanced by Dr. Singer's development of imaging technology and RNA reporters. Dr. Singer's laboratory has been instrumental in developing rapid and sensitive microscopy to study single molecules of RNA in living cells to track them through their life cycle. Using these technologies, he can observe single mRNAs as they are transcribed and processed, as they export from the nucleus, localize to cytoplasmic compartments such as dendritic processes of neurons, and finally when and where they translate and are degraded. RNA Localization: Following Single mRNAs from Birth to Death in Living Cells, Click this link to view the Singer Lab Publications. A patented Dr. Robert H. Singer received his undergraduate degree in physical chemistry from Oberlin College and his Ph.D. in developmental bi His career has been focused on the cell biology of RNA, its isolation, detection, expression and translation. By making transgenic, knock-in mice where the endogenous RNA is tagged with the stem loops of the MS2 phage, and bound to the cognate fluorescent coat protein, these events can be seen in living tissues, in order to ultimately understand the native regulatory mechanisms governing gene expression. Studying Single mRNA Molecules from Cradle to GraveWe seek to understand the expression and movement of mRNA from transcription through degradation and the effect that defects in these processes have on health. © 2020 Albert Einstein College of Medicine Copyright © 2020 National Academy of Sciences. Policy on Discrimination, Harassment, and Bullying, Proceedings of the National Academy of Sciences. Science interviews Robert Singer, Ph.D., who is identified as a pioneer in the field of mRNA research, about the ongoing search to discover how proteins are placed in their correct destinations within a cell.

Edit profile. In addition, Dr. Singer’s laboratory has been instrumental in developing rapid and sensitive microscopy that can study single molecules of RNA in living cells and in devising methods to track them through their life cycle. He holds 12 patents on his work. Dr. Singer is professor and co-chair of anatomy & structural biology and co-director of the Gruss-Lipper Biophotonics Center at Einstein. Using these technologies, we can observe single mRNAs localizing to cytoplasmic compartments such as the leading edge of a fibroblast, the bud tip of yeast or the axonal process of neurons and follow these single mRNAs from transcription, nuclear export through translation and degradation. Robert H. Singer is the Harold and Muriel Block Chair of Anatomy of Structural Biology, a Professor of Cell Biology and Professor of Neuroscience. 1300 Morris Park Avenue Bronx, NY 10461 He develops methods to label RNA in fixed and living cells using fluorescent probes and the microscopy techniques and image analysis algorithms to visualize and quantify many mRNAs simultaneously. people_blocks-people_blocks_name_and_labs | block, people_blocks-people_blocks_action_picture | block, people_blocks-people_blocks_bio_summary | block, people_blocks-people_blocks_biography | block, janelia7_blocks-janelia7_featured_blocks | block, 19700 Helix Drive, Ashburn, Virginia 20147. He then did postdoctoral work in molecular biology at MIT and the Weizmann Institute in Israel.